Induction of the copper resistance operon from Pseudomonas syringae

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Copper Hypersensitivity and Uptake in Pseudomonas syringae Containing Cloned Components of the Copper Resistance Operon.

Copper resistance in Pseudomonas syringae carrying the copABCD operon is associated with accumulation of copper in the periplasm and outer membrane, apparently as a function of the copper-binding activities of the copABC gene products. However, no specific function for copD has been determined. In this study, P. syringae cells containing copCD or copBCD cloned behind the lac promoter were hyper...

متن کامل

A two-component regulatory system required for copper-inducible expression of the copper resistance operon of Pseudomonas syringae.

Specific induction of the copper resistance operon (cop) promoter from Pseudomonas syringae was measured by beta-galactosidase production from a cop promoter-lacZ fusion. Induction of the cop promoter in P. syringae pv. syringae required trans-acting factors from copper resistance plasmid pPT23D, from which cop was originally cloned. Tn5 mutagenesis of pPT23D was used to localize two complement...

متن کامل

Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae.

Strains of Pseudomonas syringae pv. syringae resistant to copper, streptomycin, or both compounds were recovered from symptomless and diseased tissue of four woody hosts in three nurseries in Oklahoma. In strains resistant to copper and streptomycin (Cur Smr), resistance to both compounds was cotransferred with a single plasmid which was either 68, 190, or 220 kilobase pairs (kb). All Cus Smr s...

متن کامل

CopC protein from Pseudomonas syringae: intermolecular transfer of copper from both the copper(I) and copper(II) sites.

The CopC protein from Pseudomonas syringae pathovar tomato is expressed as one of four proteins encoded by the operon CopABCD that is responsible for copper resistance. It is a small soluble molecule (10.5 kDa) with a beta-barrel structure and features two distinct copper binding sites, which are highly specific for Cu(I) (K(D) > or = 10(-)(13)) and Cu(II) (K(D) approximately 10(-)(15)). These ...

متن کامل

Genetic Basis of Copper Resistance in New Zealand Strains of Pseudomonas Syringae

Strains of Pseudomonas syringae able to grow on minimal medium containing 500 mg/litre of copper sulphate were selected from a collection of streptomycin-resistant strains isolated in New Zealand. From one of these copper-resistant strains a 1.3 kb fragment of DNA was isolated by PCR using primers designed on genes reported to be associated with copper resistance. Sequencing of this fragment re...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1988

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.170.9.4399-4401.1988